Screening of Probiotics Efficiency to Inhibit Persistent Biofilm-Related Infections

Abstract

Abstract: Probiotics are live bacteria. They are administered in food, with beneficial effects to the host, help to prevent or treat diseases, the majority of which are gastrointestinal. The isolation and utilization of Lactobacillus spp. as probiotics from dairy or some other items has been exhibited to be health promoting. Modification in Gut flora contribute in different sorts of disease conditions, and distinctive Microorganisms developing in a biofilm are related with constant and chronic human diseases which are resistant to antimicrobial agents. The biofilm producing organisms are E.coli, Pseudomonas, Klebsiella, Staphylococcus and many more. Objective: The aim of study is to screen the efficiency of probiotics in the inhibition of bacterial biofilm. Methodology: Two methods used for screening of biofilm production are, Tube method and Congo red method. Three species of Lactobacillus
were isolated and screened for biofilm inhibition. Four commercial isolates of E. coli were obtained and among those, three were efficiently able to produce biofilm. All species of Lactobacillus were able to counteract the biofilm production. Results: Lactobacillus species were identified to be L. plantarum, L. casei and L. bulgaricus on the basis of sugar test and microscopy, which inhibited the biofilm formation of intestinal bacteria. Conclusion: After this study there is no doubt in believing that the Probiotics are definitely new hope towards treatment of infections that are prevailed among people. Conventional dairy items are great source of bacterial strains and bioactive compound discharged by these three identified strains could be utilized in pharmaceuticals for recovery from bacterial diseases.