LABs Responsible for Enhancing Antibiotic Susceptibility Pattern and Gene Transference

Abstract

ABSTRACT: Probiotics are gram positive organisms considered beneficial for combating with various pathogens. Most of
them are antibiotic resistant, giving an idea that they may be carrier of r-plasmids. Our aim of the study was to determine the
proportion of LABs that associate with r-gene transference as well as in boosting up antibiotic susceptibility range. For that we
isolated probiotic cultures of Streptococcus, Lactobacillus, and Leuconostoc from milk sample, and checked the susceptibility
pattern of isolated probiotics and pathogens (test organisms) against carbepenem. Results showed all LABs are resistant, while
most of the pure test organisms were sensitive, after that these pathogens were treated with Labs coated disc that was prepared
by the Kirby Bauer method. These LAB treated organisms were again checked with carbepenem to check the susceptibility
pattern, this whole protocol was carried out on Muller Hinton Agar (MHA) plate. According to our results test organisms
treated with Streptococcus and Lactobacillus species occurred with a 20% and 1% of resistivity, while organisms treated with
Leuconostoc species enhanced the sensitivity 66% and streptococcus 1%, whereas Lactobacillus did not show any change in
antibiotic spectrum.