Efficacy of Hematological Indices for ß Thalassemia Trait Screening in Pregnant Women


  • Maryam Habib Department of Hematology, Faculty of Pathology, Shifa College of Medicine (STMU) Islamabad, Pakistan.
  • Masooma Shaheen Department of Pathology, Tehsil Headquarter Hospital, Attock, Pakistan.
  • Amina Nazir Department of Pathology, District Headquarter Hospital, Chakwal, Pakistan.
  • Wardah Aslam Department of Hematology, Faculty of Pathology, NORI Hospital, Islamabad, Pakistan.


ß Thalassemia trait, Hemoglobin electrophoresis, Red cell indices, Microcytic anemia, Iron deficiency


Abstract: Background: ß thalassemia is one of commonest inherited disorder in Pakistan having a carrier rate of 5%. Pregnant women having ß thalassemia trait can have offspring having ß thalassemia major which is lifelong blood transfusion dependent state. Different screening programmes are being carried out in various parts of the world so that the birth of children having ß thalassemia major can be effectively reduced. Objective: To determine the diagnostic accuracy of hematological parameters in identifying ? thalassemia trait in pregnant women keeping measurement of HgbA2 as gold standard. Methods: 108 pregnant women were enrolled during one year of the study period. Blood sample was taken and blood complete picture was obtained. By using variables of CP card Green & King index, Shine & Lal index and RDWI were calculated keeping hemoglobin electrophoresis as gold standard. Results: The age of patients ranged from 17 to 37 years with mean age of 24.65. Out of 108 pregnant women enrolled in study 40 were found to have ß thalassemia trait based on results of hemoglobin electrophoresis. Shine & Lal index showed 100% sensitivity, 0% specificity, 35% PPV and 0% NPV. Green & Kings index had sensitivity of 32%, specificity 100%, PPV of 100% and NPV of 71% while RDWI showed 50% sensitivity, 100% specificity, PPV of 100% and NPV of 77% in diagnosing ß thalassemia trait in pregnant women. Conclusion: Automated cell counters based formula including Green & King index, Shine &Lal index and RDWI provide rapid, reliable and cost effective method for screening of ß thalassemia trait especially in third world countries like Pakistan. However none of them have 100% sensitivity and specificity. So they should be collectively looked at in screening programmes.




How to Cite

Habib M, Shaheen M, Nazir A, Aslam W. Efficacy of Hematological Indices for ß Thalassemia Trait Screening in Pregnant Women. Nat J Health Sci [Internet]. 2020Dec.29 [cited 2024May20];2(4):155-8. Available from: https://ojs.njhsciences.com/index.php/njhs/article/view/111



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